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Bioseparationsprinciplesandtechniquesbybsivasankarebookfreedownload wiltho



 


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chapter11.shtml ISBN: 978-93-5262-144-3 **Purification of Polyphenol-Rich Extracts from Wild Berries by Antioxidant Profiling and Determination of Their Antioxidant Properties** By Vinay Kumar Ghimire Abstract Antioxidant characterization and quantification of polyphenols, flavonoids, and anthocyanins by HPLC-DAD method were developed. Various wild berries of Nepal including blackberry, bilberry, and strawberry were collected from the Himalayan region. Flavonoids, anthocyanins, and polyphenols were found to be higher in various wild berries, with high-bush blueberry and strawberry being the most abundant. The antioxidant activity of different polyphenols, flavonoids, and anthocyanins was evaluated by the DPPH, FRAP, and ABTS radical scavenging activity assays. Finally, the relationship between the type and concentration of antioxidants and their antioxidant activities was evaluated. All antioxidants showed strong antioxidant activity. The results also showed a significant correlation between antioxidant and polyphenol contents. The findings of the study may be useful for better understanding the antioxidant activity of various wild berries. Keywords Wild berriesNepalPolyphenolFlavonoidAnthocyaninAntioxidant activityDetermination profileDetermination assayDPPHFRAPABTS ## **1 Introduction** Medicinal herbs have been used to treat disease for a long time. Despite the extensive use of medicinal herbs, however, many of them have gone into oblivion. Some of the reasons for this are low pharmaceutical yield, complex processing of medicinal herbs, and difficulties in standardization of their active constituents. Nowadays, the demand for natural products is on the rise, and therefore, natural products obtained from the pharmaceutical industry are being replaced by those of natural origin. Indeed, plants have been used to treat many diseases and disorders since ancient times. Medicinal plants have been used as food, medicine, or traditional medicine for a long time, and they are still widely used. Since the development of modern science, the role of medicinal plants and herbal extracts in the prevention and treatment of disease is being thoroughly researched. In the past, it was believed that there were no medical benefits associated with medicinal herbs, but now their importance has been recognized by the medical community. Today, there is growing evidence of medicinal herbs as potential remedies for various conditions, such as diabetes, cancer

 

 

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Bioseparationsprinciplesandtechniquesbybsivasankarebookfreedownload wiltho

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